首页> 外国专利> A PROCESS OF ISOLATION OF SNAKE VENOM PLA2S, AND THE RESULTANT PLA2S AND/OR A PHARMACEUTICAL COMPOSITION THEREOF FOR THE TREATMENT OF HYPERLIPIDEMIA

A PROCESS OF ISOLATION OF SNAKE VENOM PLA2S, AND THE RESULTANT PLA2S AND/OR A PHARMACEUTICAL COMPOSITION THEREOF FOR THE TREATMENT OF HYPERLIPIDEMIA

机译:蛇毒PLA2的分离工艺,所得的PLA2和/或其药物组合物,用于治疗高脂血症

摘要

The invention relates to isolation of PLA2s from snake venom and it uses in the treatment of hyperlipidemia either individually or in combination. The basic snake venom used for the process is selected from V. russellil venom and N. naja venom. The isolation process comprise of a) fractionating the venom on chromatography using suitable molarities of phosphate buffer solution to result in multiple fractions b) selecting the fraction containing PLA2, Lyophilizing the same and further subjecting to chromatography and eluting stepwise using phosphate buffers of varying molarities and pH (0.05 M - 0.2 M, pH 7.0-pH 8.0) resulting into two fractions; c) selecting the major fraction and subjecting to chromatography and eluting using the suitable molarities of phosphate buffer pH 7.0 resulting in or pre equilibrated with 0.1 M NaCI, resulting in homogenous PLA2s. The isolated snake venom PLA2s is converted to a pharmaceutical composition by conjugating with Nano gold /iron particles or for effective and site directed delivery.
机译:本发明涉及从蛇毒中分离PLA 2,并且其单独或组合用于治疗高脂血症。用于该过程的基本蛇毒选自V. russellil毒和N. naja毒。分离过程包括:a)使用合适摩尔浓度的磷酸盐缓冲溶液在色谱上分离毒液,得到多个馏分b)选择含有PLA2的馏分,将其冻干,然后进行色谱分离,并使用不同摩尔浓度的磷酸盐缓冲液逐步洗脱。 pH(0.05 M-0.2 M,pH 7.0-pH 8.0)分为两部分; c)选择主要馏分,进行色谱处理,并使用适当摩尔浓度的磷酸盐缓冲液pH 7.0洗脱,得到0.1 M NaCl或将其预先平衡,得到均质的PLA2。通过与纳米金/铁颗粒结合或用于有效和定点递送,将分离的蛇毒PLA2s转化为药物组合物。

著录项

  • 公开/公告号IN2011CH01662A

    专利类型

  • 公开/公告日2013-06-21

    原文格式PDF

  • 申请/专利权人

    申请/专利号IN1662/CHE/2011

  • 发明设计人

    申请日2011-05-16

  • 分类号

  • 国家 IN

  • 入库时间 2022-08-21 16:41:23

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