A PROCESS OF ISOLATION OF SNAKE VENOM PLA2S, AND THE RESULTANT PLA2S AND/OR A PHARMACEUTICAL COMPOSITION THEREOF FOR THE TREATMENT OF HYPERLIPIDEMIA

摘要

The invention relates to isolation of PLA2s from snake venom and it uses in the treatment of hyperlipidemia either individually or in combination. The basic snake venom used for the process is selected from V. russellil venom and N. naja venom. The isolation process comprise of a) fractionating the venom on chromatography using suitable molarities of phosphate buffer solution to result in multiple fractions b) selecting the fraction containing PLA2, Lyophilizing the same and further subjecting to chromatography and eluting stepwise using phosphate buffers of varying molarities and pH (0.05 M - 0.2 M, pH 7.0-pH 8.0) resulting into two fractions; c) selecting the major fraction and subjecting to chromatography and eluting using the suitable molarities of phosphate buffer pH 7.0 resulting in or pre equilibrated with 0.1 M NaCI, resulting in homogenous PLA2s. The isolated snake venom PLA2s is converted to a pharmaceutical composition by conjugating with Nano gold /iron particles or for effective and site directed delivery.