MYCOSYNTHESIS OF SILVER NANOPARTICLES USING EDIBLE MUSHROOM PLEUROTUS FLORIDA AND ITS USE AS ANTIBACTERIAL, ANTIFUNGAL AND APOPTOTIC AGENT.

摘要

ABSTRACT OF THE INVENTION: • The innovation of Mycosynthesis with silver nanoparticles using cell filtrate extract of edible mushroom Pleurotus florida is a clean, nontoxic and go green method which is in great demand for the society. • The above mentioned innovative method is evidenced with spectrophotometry, for characterization of the particles scanning and transmission electron microscopy for size and shape of the particles. The size ranged between 10-57 nm in diameter. • The mycosynthesis using edible mushroom are naturally protein coated silver nanoparticles which facilitates drug delivery. • The silver nanoparticles produced in this invention had potent antimicrobial effect on human bacteria E. coli (DH5α), plant pathogenic bacteria Agrobacterium tumefaciens (LBA4404) and the human pathogenic fungus Aspergillus sp and on the multi drug resistant E. coli and Agrobacterium tumefaciens. These can also inhibit the spore germination of plant pathogenic fungus Alternaria solani thereby exploring scope of formulating pesticides. • Single cell gel electrophoresis was done on tobacco and tomato leaf nuclei which showed apoptogenic activity at higher concentrations. So this can be used as an anti-tumor agent. • So engineered silver nanoparticles can be used in early detection of diseases and most importantly, molecular targeted therapy, have the potential to revolutionize the diagnosis and treatment of many bacterial and fungal diseases