EXOFLAP CLONING-A NOVEL, SIMPLE AND EFFICIENT LIGATION-INDEPENDENT CLONING TECHNIQUE USING TAQ DNA POLYMERASE

摘要

Provided herein are methods of directional cloning of DNA/cDNA using Taq DNA polymerase using ExoFlap cloning. ExoFlap cloning makes use of flap endonuclease activity and 5" - 3" Exonuclease of Taq DNA polymerase.