METHOD FOR OBTAINING RECOMBINANT PROTEINS WITH IMMUNOGENIC CAPACITY OF PICHIA PASTORIS AND USE THEREOF IN A PREPARATION OF VACCINES AGAINST INFLUENZA VIRUS.

摘要

The present invention pertains to the genetic engineering and biotechnology field and particularly refers to the production of vaccines against influenza virus AH1N1, the invention referring to a method for producing recombinant proteins with immunogenic capacity of Pichia Pastoris and the use thereof in vaccines against the influenza virus, said method comprising the phases of: obtaining, by molecular engineering, a first and second sequence of recombinant AND, which provides Pichia pastoris with the capacity of producing viral chalcogens of Influenza AH1N1 MX 2009; integrating each sequence of recombinant ADN of the vectors pPIC9:HA30762 and pPIC9:HA30780, obtained in the phase I, to the genome of Pichia pastoris for obtaining a first and second strain of recombinant Pichia pastoris able to produce a viral antigen per vector against AH1N1 Mexico 2009; producing a first and second recombinant viral antigen of influenza AH1N1 Mexico 2009 in the recombinant Pichia pastoris obtained in the phase II.