Method and set for detection of increased hereditary predispositions towards cancer of various organs through identification of germinal mutation within the CHEK2 gene

• 1. 遗传性和易感性线粒体耳聋基因检测的试剂盒 [P] . 中国专利： CN107988357A . 2018-05-04
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• 3. METHOD AND SET FOR DETECTION OF INCREASED HEREDITARY PREDISPOSITIONS TOWARDS CANCER OF VARIOUS ORGANS THROUGH IDENTIFICATION OF GERMINAL MUTATION WITHIN THE CHEK2 GENE [P] . PL367319A1 . 2005-10-17

  机译： 通过CHEK2基因内的突变鉴定来检测各种有机物对癌症的遗传倾向增加的方法和装置

• 4. Method and kit for the detection of increased hereditary predisposition to cancers of various organs through the identification of germline mutations in CHEK2 gene [P] . PL214831B1 . 2013-09-30

  机译： 通过鉴定CHEK2基因种系突变来检测各种器官的遗传易感性的方法和试剂盒

• 5. Method and kit for the detection of increased hereditary predisposition to cancers of various organs through the identification of germline mutations in CHEK2 gene [P] . PL399115A1 . 2012-10-22

  机译： 通过鉴定CHEK2基因种系突变来检测各种器官的遗传易感性的方法和试剂盒

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• 1. A Simple LDT-PCR Method for Detection of Various Nucleic Acid Sequence Changes in a Small Region of Genes—Application for the Identification of Gene Mutations in MTB irpoB/iGene Associated with Drug Resistance [J] . Xing Tang ,Steven Wood ,Larissa Baeva . 医学微生物学（英文） . 2013,第3期
• 2. A Thai family with hereditary pancreatitis and increased cancer risk due to a mutation in PRSS1 gene [J] . Theeraphong Pho-Iam ,Wanna Thongnoppakhun ,Pa-Thai Yenchitsomanus . 世界胃肠病学杂志：英文版 . 2005,第11期
• 3. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 4. Detection of hMSH2 and hMLH1 mutations in Chinese hereditary non-polyposis colorectal cancer kindreds [J] . Chang-Hua Zhang ,Yu-Long He ,Fang-Jin Wang . 世界胃肠病学杂志：英文版 . 2008,第2期
• 5. 采用多种检测方法综合确定PHC桩补强方案 [C] . 黄阳 ,韩高升 ,柳春 . 第七届全国桩基工程学术年会 . 2005
• 6. FEN1基因的遗传学和表观遗传学调控及其与多种肿瘤发生发展的关系 [A] . 杨明 . 2008