a new gene coding for a lipase of talaromyces thermophilus and a process for synthesis of bio fuel additive in the immobilisee lipase.

摘要

this patent concerns the isolation of the total lipase gene of a new strain of thermophilic fungus talaromyces thermophilus. the gene consists of an open reading phase of 1080 bp, interrupted by three introns of small sizes and encodes a mature protein with a molecular mass of 269 aa theory about 29590 da.the catalytic site is represented by the triplet, ser, glu, his, as well as the motive characteristic x x x x x x x x x x x x x x ser gly gly ser - surrounding the catalytic lipase is well preserved. this enzyme belongs to the family of mucorales lipases.bioinformatics analysis of the promoter region of this gene was used to predict the basal promoter sequences in boxes tata, caat, etc., a number of potential sites are not specific lipases rec, cap c and eta 2 known to regulate the expressi we selected protein, respectively, depending on the source of carbon, ph, and oxygenation.other consensus sequences for the binding of specific transcription factors have been identified, such as lipases also do analysis by southern blot and in conditions stringentes, t. thermophilus genomic dna (indicates the presence of a single gene is best ten wheat bran as substrate, olive oil, conventional induction of lipases.this lipase is thermo alkaline and has a good stability to thermal denaturation, it maintains more than 90% and 60% of its activity after incubation for 60 min, and only particles, respectively. the immobilization of the enzyme by covalent linkage of chitosan resulted in the improvement of its heat stability.the immobilized lipase was used in estu00e9rifications reactions for the synthesis of an additive used in biofuels, butyl oleate. this enzyme can be used to achieve transu00e9tu00e9rification reactions (reaction of an ester and an alcohol and (2 interu00e9stu00e9rification reaction of esters).