synthesis process of bioplastic enzymatically biodegradable plastic from renewable resources (e.g., starch and fatty acid, and lipase as biocatalyst using staphylococcus aureus immobilisee on caco3.

摘要

the present invention relates to the enzymatic synthesis of a biopolymer (esters of starch based biodegradable plastic starch) or (bio plastics to replace the ordinary plastic harmful to health and the environment. plastics are polymers produced from renewable biological resources (starch, cellulose. .).these polymers are used in a variety of areas: pharmaceutical, medical and food packaging and biodegradable.the bioplymu00e8re synthesized in the present invention has been achieved by direct esterification between corn starch and oleic acid by using lipase as biocatalyst, staphylococcus aureus (sal3) immobilized by adsorption on calcium carbonate (sal3i).in contrast to chemical synthesis, which operates in harsh conditions, the enzymatic synthesis reactions are more selective and can be implemented under mild conditions of temperature and pressure.the conditions of synthesis, namely, the amount of enzyme, temperature, the ratio of starch of oleic acid, the effect of the addition of organic solvents and the amount of molecular sieves have been optimized. the performance of synthesis of biopolymer (esters of starch in the sal3i, under optimal conditions, is 75% with a degree of substitution of 2.86.analysis by thin layer chromatography (tlc), the products of the hydrolysis of native starch and starch esterified by porcine pancreatic amylase (ppa) shows that, in contrast to the native starch, esterified starch (biopolymer) is not hydrolyzable by the alpha amylase activity.this loss of digestibility due to the hydrophobic nature of the biopolymer produced by the esterification of oleic acid and starch. the modification of the structure of the starch ester bond formation with oleic acid (esters of starch has been confirmed by three measures: nmr, dsc (differentiai scanning calorimetry) and viscosity.after the esterification reaction of starch enzyme, semi crystalline state is a (directed) as the lens (not ordered). the biopolymer is described: (1) contains a quantity of heat flux (u0394h) 101.1 j / g compared to native starch 375j / g), and (2) a viscosity of 84.5 mpa. s at 5 mpa. s to native starch.key words: lipase immobilization on staphylococcus aureus, caco3, oleic acid, starch, enzyme catalyzed esterification, biopolymer, biodegradable packaging.