PROCESS FOR PRODUCTION OF NUTRIENT AGAR MEDIUM FOR INVESTIGATION OF GRANULOCYTE-MACROPHAGE COLONY-FORMING ACTIVITY OF HEMOPOIETIC STEM CELLS

摘要

A process for production of nutrient agar medium for investigation of granulocyte-macrophage colony-forming activity of hemopoietic stem cells comprises preparation of nutrient medium by mixing of nutrient medium with embryonal veal serum, vitamins, replaceable and irreplaceable amino acids, sodium pyruvate, L-glutamine, addition of melted agar, and mixing. 10-fold concentrate of Igla medium MEM is used as nutrient medium, and HEPES-buffer is additionally introduced, with subsequent mixing of one part of 2-fold concentrate of nutrient medium, heated up to , with one part of 0.6% agar solution of the same temperature.