Method of expansion / conservation of undifferentiated hemopoietic stem cells or progenitor cells, method of preparing a conditioned stomach cell medium useful in the expansion / conservation of undifferentiated hemopoietic stem cells or progenitor cells, method of transplanting undifferentiated hemopoietic stem cells or progenitor cells into a container , buffer of bioreactor and bioreactor

摘要

"METHOD OF EXPANSION / CONSERVATION OF CELLS DETRONCO hemopoietic undifferentiated OR DASCéLULAS PROGENITOR, METHOD OF PREPARATION DEUM CONDITIONING HALF cell STOMACH USEFUL NAEXPANSãO / CONSERVATION OF TRONCOHEMOPOIéTICAS CELLS undifferentiated OR THE CéLULASPROGENITORAS, METHOD CéLULASDE TRANSPLANTATION BODY hemopoietic undifferentiated OR DECéLULAS PROGENITOR IN A CONTAINER, DEBIORREATOR AND BIORREATOR CAP ". Understanding a method of expanding / maintaining undifferentiated hemopoietic stem cells or stem cells by obtaining undifferentiated stem cells or hemopoietic stem cells; and seeding either undifferentiated stem cells or stem cells in a stationary phase flow bioreactor in which a three-dimensional culture of stromal cells has previously been established on a slide-shaped substrate, including the substrate a fibrous non-woven matrix forming a three-dimensional network of physiologically acceptable fibers, thereby expanding / maintaining undifferentiated stem cells or hemopoietic progenitor cells or culturing stem cells in hematopoietic indifferentiated hemopoietic stem cells. a conditioned medium obtained from such a reactor.