Improved performance in protein expression systems of protein synthesis in the absence of cells by adding antifoams

摘要

A process for the in vitro translation of mRNA in the absence of cells to producirpolipéptidos, the process comprising the synthesis of said polypeptide in a reaction mixture enausencia cell with more than 15 ul volume, comprising a separate antifoam undetergente in a concentration of at least 0.00007%, and not more than 0.007% by weight wherein the agenteantiespumante is selected from block copolymers, which provide defoaming / antifoaming action by forming an insoluble monolayer at the interface air / water the foam; dealquilpolioxialquileno glycol; siloxane polymers; and mixtures of polyether-based dispersions polipropilenoorgánico not siliconized.