Bioengineered tissue constructs and methods modified to produce and use

摘要

A tissue construct grown prepared by crecUn tissue construct cultivated prepared by growing fibroblast cells under condicionesimiento fibroblast cell under conditions to produce a layer of extracellular matrix to produce a layer of extracellular matrix which is synthesized and assembled by cells fibrob is synthesized and assembled by fibroblast cells, with the cultured fibroblast cells clast with the cultured fibroblast cells contained in the extracellular matrix layer sintetontenidas in the synthesized extracellular matrix layer, wherein the fibroblast cells are cultivaizada where cells fibroblast cultured in a chemically defined medium free Extran in a chemically defined medium free tissue extracts or animal organs undefined undefined tissue or animal organs cts and wherein the cell culture medium chemically dy where the cell culture medium q uímicamente defined comprising: (i) nutrient base medium; (Iefinido comprises: (i) nutrient base medium; (ii) insulin, (iii) L-glutamine or L-alanyl-L-glutami) insulin; (Iii) L-glutamine or L-alanyl-L-glutamine; and (iv) ascorbate or a derivative ina ascorbate; and (iv) ascorbate or a derivative ascorbate and where fibroblast cells produce maty where fibroblast cells produce said extracellular matrix in the absence of extracellular componeriz mentioned in the absence of exogenous matrix components or synthetic members Duntes exogenous matrix or synthetic members during the culture conditions. Rante culture conditions.