Polypeptide with phytase activity and increased thermal stability of the enzyme activity and a nucleotide sequence encoding it

摘要

Recombinant DNA molecule, which upon expression in a prokaryotic host cell or eukaryotic, encoding a polypeptide having phytase activity, containing the recombinant DNA molecule one deADN sequence, which is selected from a) DNA sequences which encoding a polypeptide having phytase activity, which obtenidomediante sequence variation of mature E. coli phytase wildtype had, lavariación comprising a combination of asparagine → arginine mutations at position 139 (N139R) and → aspartic acid glutamic at position 142 (D142E), b) acid DNA sequences which, due to the degeneracy of the genetic code, are related with lassecuencias according to a), performing the recombinant DNA molecule, in the case of expression in a suitable host cell, it is accompanied by a thermal stabilities and against proteases increased enzymatic activity protein encoded thereby.