IMMORTALIZED, HOMOZYGOUS STAT1-DEFICIENT MAMMALIAN CELL LINES AND THEIR USES

摘要

The present invention is directed to immortalized STAT1-deficient mammaliancell lines. STAT1 is a signal transducer and activator of transcription thatbecomes phosphorylated when cells are treated with type I or type IIinterferons and leads to induction of specific gene expression, resulting inestablishment of the antiviral state and the other known biological responsesto interferons, including the inhibition of cell proliferation. Cells whichlack this gene product are useful for producing high titers of viral stocks,for producing recombinant viral vectors, for testing samples, especiallyclinical samples for the presence of virus and for screening candidatecompounds or drugs for anti-viral activity.