SELECTION OF ALLOSTERIC REGULATORS WITH CLASS III G PROTEIN-COUPLED RECEPTORS DEPLETED OF THE EXTRACELLULAR DOMAIN

摘要

The present invention relates to a method for identifying and selecting a compound acting as an allosteric regulator of a GPCR-III. According to this method, the level of activity of a recombinant RCPG-III (RCPG-III-DELTA.), Essentially devoid of the corresponding native RCPG-III extracellular domain, in the presence of a given compound, is compared with that the same RCPG-III-.DELTA., in the absence of said compound. This method thus makes it possible to identify and select positive or negative allosteric regulators of GPCR-IIIs. The invention also aims to apply such a method to the identification and selection of compounds of interest in fields of activity such as pharmacy, the food industry, the environment, the ecology and basic research.