NUCLEASE-RESISTANT RNA APTAMER INHIBITING REPLICATION OF HEPATITIS C VIRUS REPLICON

摘要

Disclosed is a nuclease-resistant RNA aptamer forinhibiting the replication of HCV replicon. This aptameris capable of binding specifically to HCV NS5B andinhibiting the proliferation of the HCV replicon, andincludes at least one sequence selected from a groupconsisting of SEQ ID NOS. 1 to 4, in which a fluoro groupis substituted for 2'-hydroxy of all of the U (uracil)bases and all of the C (cytosine) bases, and SEQ ID NO.17, in which the fluoro group is substituted for the2'--hydroxy of all of the U (uracil) bases and all of the C(cytosine) bases, and which is tagged with a cholesterylgroup at a 5' end and with idl at a 3' end. The RNAaptamer is useful in the diagnosis and treatment of HCVinfection.