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USE OF TAQ POLYMERASE MUTANT ENZYMES FOR DNA AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS
USE OF TAQ POLYMERASE MUTANT ENZYMES FOR DNA AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS
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机译:在存在PCR抑制剂的情况下使用TAQ聚合酶突变酶进行DNA扩增
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摘要
The present invention generally relates to gene detection in standard and real-time PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time polymerase chain reaction (PCR) assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.
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