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Use of TAQ polymerase mutant enzyme for DNA amplification in the presence of PCR inhibitors
Use of TAQ polymerase mutant enzyme for DNA amplification in the presence of PCR inhibitors
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机译:在PCR抑制剂存在下TAQ聚合酶突变酶在DNA扩增中的用途
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摘要
The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.
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