Methylglyoxal Fortified Honey The invention describes a method and process whereby the antibacterial properties of honey or a product containing predominantly honey are increased by the addition of methylglyoxal thereby creating a Methylgyoxal Fortified Honey product.

摘要

#$%^&*AU2013100967A420130919.pdf#####Abstract The invention provides antimicrobial compositions comprising 1,2-dicarbonyl compounds such as 3deoxyglucosone (3-DG, alternatively 3-deoxyglucosulose) and/or glyoxal (GO) and/or methylglyoxal (MGO), preferably MGO added to honey such as Manuka honey derived from myrtacae family preferably Manuka tree (Leptospermum scoparium). The invention provides a method of fortifying or increasing levels or enriching methylglyoxal in honey by addition of methylglyoxal optionally with or without one or more antibacterial acyclic 1,2-dicarbonyl compounds to honey. The method and methylglyoxal fortified honey composition results with increased antibacterial properties. The invention provides antimicrobial composition for therapeutic use against Escherichia coli (E. coli) or Staphylococcus aureus (S. aureus), moreover applied to wounds as remedy for bacterial infections. The invention describes a method and process whereby honey that may or may not contain methylglyoxal is fortified with the addition of methylglyoxal, thereby increasing the antibacterial property of that honey or product containing predominantly honey. The present invention describes a product containing Honey to which Methylglyoxal, also called pyruvaldehyde or 2-oxopropanal (CH3-CO-CH=O or C3H402) is added. The present invention also describes a product composed predominantly of Honey fortified with methylglyoxal during the production process. The present invention also describes product composed predominantly of Honey that contains naturally derived methylglyoxal that is fortified with additional methylglyoxal during the production process. The production process involves either adding or mixing or absorption of MGO with honey.DRAWINGS PAGE ONE OF FOUR DRAWINGS DRAWINGS AND GRAPHS Table 1. Sample 3-4G. Go MGM Commercialhoney 342 1.7 3A 3-9 samples (n=50) (119-1451) (nd. -4.6) (n.d,-5-7) (1.0-75) Manuka1 "active 5" 1060 54 07 ±0.2 38.4±5,0 ±0,0.2 Manuka active" 6C8 30 3 0* 1.0 347±20 22.6 t 0.5 Manuka3 "active 563 i26 3-9 1.0 411 24 17.6 0.6 Manuka4"UMF 10" 747±40 1.2 0.5 41635 213 1.1 Manuka5 'UMF:20" 807*39 4,2 1 1 743± 40 43.9. *2.0 Manuka.6 "UMF 25- 697 t 44 7.0.t 761 ,25 n.a. Antibacterial wound gel n.a. n.a. 312 20 n.a. Table 2. Sample MIC for E coll MIC for S aureus 3-DG No inhibition observed up to 60 mM GO 6.9 mM 4,3 mM MGO 1.1 mM Honey samples 80% Manuka honeys 2-6 15-30% MGO at MIC dilution 1.1 -1.8 mMDRAWINGS PAGE TWO OF FOUR [mA-LI, so A.. Standard 4 0 - "6 1B. Forest honey 4w00 CLi 0m 0 0 10I 20 40 MG (45 Mnj(L nUBIfoes hoey C auahn