Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers

摘要

#$%^&*AU2013203624A120130502.pdf#####ABSTRACT [00101] Methods and kits for amplifying a nucleic acid under isothermal conditions to form an amplified nucleic acid sequence are provided. The methods and kits comprises providing a nucleic acid template, a DNA polymerase, deoxyribonucleoside triphosphates, a primer comprising a randomized sequence, and a specific primer, and amplifying the nucleic acid template.1/7 FIG. 1 Circular DNA Circular RCA DNA product Phi 29 Random primers Extension Random primers polymerase Specific Specific - hybridization primers bead-1 bead-1 Specific primers