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HIGH-THROUGHPUT AND NON-INVASIVE METHOD TO VITRIFY PORCINE EMBRYOS
HIGH-THROUGHPUT AND NON-INVASIVE METHOD TO VITRIFY PORCINE EMBRYOS
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机译:高通量和非侵入性的方法对猪胚进行玻璃化
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摘要
The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the IVP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.
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