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METHOD FOR DETERMINING THE SENSITIVITY OR RESISTANCE OF RETROVIRUS ISOLATES TO MOLECULES AND DIAGNOSTIC KITS
METHOD FOR DETERMINING THE SENSITIVITY OR RESISTANCE OF RETROVIRUS ISOLATES TO MOLECULES AND DIAGNOSTIC KITS
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机译:测定反转录病毒分离株对分子和诊断试剂盒的敏感性或抗性的方法
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摘要
A method of determining sensitivity or resistance of isolates of HIV retroviruses to a molecule includes a) amplifying sequences coding for a protease of a retrovirus to be studied, with or without the or some of amino acid sequences situated upstream and downstream of a cleavage site of a precursor in which the amino acid sequences are situated, b) recombining fragments of DNA, a final product of the amplification, and an expression vector allowing expression of sequence coding for the protease of the retrovirus to be studied under control of a known inducible promoter through co- transformation of the vector and the DNA fragments with at least one yeast cell, c) culturing co-transformed yeast cell or cells to obtain a sufficient number of transformants to perform a sensitivity or resistance test, and recovering transformants issuing from the co-transformed cell, on any suitable medium, d) incubating the transformants in the presence of a molecule to be tested, e) qualitatively or quantitatively analyzing the living cells, and f) deducing the sensitivity or resistance phenotype.
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