ANTI-BFGF HUMANIZED DOUBLE-STRANDED ANTIBODY WITH STABLE DISULFIDE BOND, PREPARATION METHOD AND APPLICATION THEREOF

摘要

Disclosed are an anti-bFGF humanized double-stranded antibody with stable disulfide bond, preparation method and applications thereof. The amino acid sequence of the anti-bFGF humanized double-stranded antibody is shown in SEQ ID NO.1. The nucleotide sequence of gene encoding the anti-bFGF humanized double-stranded antibody is shown in SEQ ID NO.2. By site-directed mutation, one cysteine residue is introduced into each single-stranded antibody, thus introduce the disulfide bond and form ds-Diabody. It is shown by experiments that the obtained ds-Diabody has the following advantages: enhanced stability; better affinity when binding to the specific antigen bFGF; moderate relative molecular weight, good tumor targeting, approved tumor tissue penetrating power, and higher blood clearance rate, showing a broad application prospects in the clinic diagnosis and medical treatment.