DIRECT REPROGRAMMING OF SOMATIC CELLS INTO NEURAL STEM CELLS

摘要

The present invention relates to a method for producing induced neural stem cells (iNSC), comprising (a) introducing into somatic cells (i) a Sox family member, (ii) a Klf family member; (iii) a Myc family member; and (iv) a POL) family member, wherein the POU family member is not Oct4; and (b) culturing the cells for at least about 2 days. The present invention further relates to induced neural stem cells obtainable by the method of the invention, as well as their use in producing induced pluripotent stem cells. Furthermore, the present invention relates to the induced neural stem cells for use in medicine or medical/pharmaceutical research, in particular for use in the treatment of a disease or disorder associated with a reduced number of neurons as compared to healthy subjects.