MAIZE AUTHENTICATION DETECTION AND MOLECULAR BREEDING SNP CHIP - MAIZESNP3072 AND DETECTION METHOD THEREOF

摘要

Disclosed is a use of 3072 SNP sites originating from maize during maize variety authentication detection and molecular breeding process. In the use of the present invention, the physical positions of the 3072 SNP sites are determined based on the whole-genome sequence alignment of the maize variety B73; the version number of the whole-genome sequence of the maize variety B73 is B73 RefGen V1; and the 3072 SNP sites are MC0001-MC3072. An experiment confirms that the authenticity of the to-be-detected maize variety can be discriminated via the comparison between the 3072 SNP sites genotype of a to-be-detected maize sample genome DNA and the 3072 SNP sites genotype of a control maize sample genome DNA. The combined use of the 3072 SNP sites of the present invention significantly improves detection accuracy and shortens detection time, thus greatly improving the maize variety detection technology level of China; in addition, the 3072 SNP sites can also be used for maize molecular breeding and providing important technical support therefor.