COST-EFFECTIVE PROCESS FOR COMMERCIAL PRODUCTION OF PACLITAXEL

摘要

Disclosed herein a cost effective, improved, continuous process for production of Paclitaxel from endophytic fungi, Fusarium solani, with substantially high yield. In an aspect, the process for the production of paclitaxel includes preparing the autoclaved culture media, preparing the inoculum followed by inoculating the inoculum on culture media and placed the culture media in the fermenter. The cells from the fermenter are harvested after 15 days. Mycelia are separated using filtration method. The mycelia is kept for drying at about 40°C and after drying crushed in Liq. Nitrogen. The solvent is added to the filtrate in the ratio of 1 :3 and kept under shaking conditions. Separating the organic layer using centrifugation and evaporating the organic solvent using rotary evaporator to obtain the dried residue of the product Paclitaxel. The dried residue is dissolved in solvent and kept overnight for evaporation until the solvent is completely evaporated.