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LIPASE INHIBITOR CONTAINING AN ECKLONIA CAVA ETHYL ACETATE FRACTIOIN OR DIECKOL ISOLATED FROM THE FRACTION
LIPASE INHIBITOR CONTAINING AN ECKLONIA CAVA ETHYL ACETATE FRACTIOIN OR DIECKOL ISOLATED FROM THE FRACTION
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机译:含有肾上腺素醋酸乙酯果胶或分离出的二氯酚的脂肪酶抑制剂
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PURPOSE: A lipase inhibitor containing an Ecklonia cava ethyl acetate fraction or dieckol isolated from the fraction is provided to ensure thermal stability and pH stability and to be used in a pharmaceutical product and health foods for suppressing obesity.;CONSTITUTION: A lipase inhibitor contains Ecklonia cava ethyl acetate fraction and dieckol as an active ingredient. A composition for suppressing obesity contains the fraction and dieckol as an active ingredient. A method for preparing the Ecklonia cava ethyl acetate fraction or dieckol isolated from the same comprises: a step of adding Ecklonia cava to a mixture solvent of water and C1-C4 alcohol and extracting at room temperature for 24 hours(three times); a step of centrifuging the extract and collecting supernatant; a step of filtering and concentrating the supernatant by reduced pressure; a step of suspending the Ecklonia cava ethanol extract in distilled water; a step of adding n-hexane, chloroform, ethyl acetate, and butanol to the extract in order; and a step of concentrating and drying the fraction.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Ecklonia cava ethanol extract; (BB) n-hexane; (CC) Hexane fraction (H); (DD,II,LL) Water layer; (EE) Chloroform; (FF) Silicagel column chromatography; (GG) Methylene chloride:ethanol; (HH) Chloroform fraction(CH); (JJ) Ethyl acetate; (KK) Ethyl acetate fraction(EA); (MM) Butanol; (NN,RR) Silicagel column chromatography; (OO) Ethyl acetate:methanol; (PP) Butanol fraction(Bu); (QQ) Water fraction(Wa); (SS) Chloroform:ethanol; (TT) Reverse phase HPLC; (UU) Solvent:36% ACN; (VV) Flux: 1mL/min, Detector: 254nm
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