Isolation and Identification of Ethyl Acetate Fractions in Flour Banana Fruit (Musa troglodytarum L.)

摘要

The use of traditional medicine is recommended by WHO to maintain of peoples health, preventing and treatment of diseases. Indonesia as a country that has biodiversity of banana, one of them is a tongka langit banana. Musa troglodytarum L. better known as banana tongka langit is one of the banana plants used by the community around Tasikmalaya to overcome various diseases such as diabetes mellitus, immune enhancers and overcome anemia. Previous research stated that the administration of ethyl acetate fraction from tongka langit banana can reduce blood glucose levels in mice induced by alloxan. Based on that, further study is needed to isolate and identify the compounds in the ethyl acetate fraction of ethanol extract of banana fruit tongka Langit (Musa troglodytarum L.). Simplicia maserated using ethanol 70%, then fractionated with LLE method using n-hexane, ethyl acetate and water, ethyl acetate fraction was isolated by using classical column chromatography and preparative thin layer method, and ensuring the isolation purity using two-way thin layer chromatography. Compound identification methods include organoleptic, thin layer chromatography (TLC) with specific spotting appearance, infrared spectrophotometry and mass spectroscopy. From the study four isolates were produced, but the MT-II-2 isolates had more results and were possible to be identified. The results of the identification group compound showed that MT-II-2 isolates were thought to be steroid. Infrared spectrophotometry results showed that isolates had functional groups C-O (Aldehyde), C-O (Carboxylate / Alcohol), C-H (Alkana), C-H Bend," C = C, C = O (Aldehyde), C-H and O-H and mass spectroscopy results that the isolate has a molecular weight of 507,1436 m / z with predictions of the molecular formulas C_(31)H_(23)O_7, C_(38)H_(19)O_2, C_(24)H_(27)O_(12).