Method for coating polyethylene glycol on surface through native chemical ligation reaction

摘要

The present invention relates to a method for coating polyethylene glycol on a surface by a natural chemical ligation reaction, and more specifically, (a) binding a phosphonic acid SAM to an oxidized or oxidized surface; (b) reacting and binding the phosphonic acid SAM with EMPSA; And (c) a method of coating polyethylene glycol on the surface including the step of reacting and binding the EMPSA with a cysteine-polyethylene glycol conjugate. The PEG coating prepared according to the method of the present invention can prevent the non-specific adhesion of samples such as proteins and cells to the surface by the non-fouling effect of PEG, so it is very effective when applied to various biological / medical equipment / devices. Provides a useful coating method.