BIOLUMINESCENT BIOSENSOR FOR DETECTING TOXIC COMPOUNDS IN A LIGNIN HYDROLYSATE

摘要

PURPOSE: A biosensor for evaluating the presence and toxicity of phenol compounds contained in a lignin hydrolysate is provided to accurately measure the presence and concentration of toxic materials without a pretreatment process.;CONSTITUTION: A gene construct contains inaA promoter(sequence number 3) of an E.coli strain BL21(DE3) and a lux gene which is operately linked to the promoter. The lux gene is lux CDABE. A biosensor for detecting a toxic material contained in a lignin hydrolysate contains the E. coli strain BL21(DE3) which is prepared through transformation by introducing the gene construct. The toxic material is furfural, benzoic acid or a salt thereof, acetic acid or a salt thereof, or phenol compounds. A method for detecting the toxic material in a sample comprises: a step of exposing the biosensor to the sample containing a toxic material; and a step of measuring bioluminescence degree.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Bioluminescence degree(A.U.); (BB) Time(min)