METHOD FOR ANALYZING VITIS VINIFERA EXTRACT

摘要

PURPOSE: A method for analyzing a Vitis vinifera extract is provided to simultaneously analyze gallic acid, procyanidin-B2, catechin, and epicatechin and to easily quantitate the extract without interference. CONSTITUTION: A method for analyzing bioequivalence of a test drug containing a Vitis vinifera extract comprises: a step of adding acid to plasma collected from an experimental subject who is administered with a test drug and mixing; a step of preparing an organic layer by liquid-liquid extraction using ethyl acetate; a step of drying the organic layer by evaporation and obtaining an analyte sample; and a step of analyzing gallic acid, procyanidin-B2, catechin, and epicatechin by a liquid chromatography-mass spectrometer.