CULTURE COMPOSITION FOR PROMOTING PHOTOSYNTHETIC MICROORGANISM AND ANIMAL CELL GROWTH WHICH ACCELERATES GROWTH AND REPRODUCTION OF MINUTE AQUATIC ORGANISMS AND A MANUFACTURING METHOD THEREOF

摘要

PURPOSE: A culture composition for promoting photosynthetic microorganism and animal cell growth and a manufacturing method thereof are provided to accelerate the production of photosynthetic microorganisms and promote growth by promoting the animal cells and reducing the stress in the cells.;CONSTITUTION: A culture composition for promoting photosynthetic microorganism and animal cell growth includes a mutant strain of Rhodobacter sphaeroides, MBTLJ- 8(accession number: KACC91572P) as an active ingredient. The generation process of the mutant strain comprises the following steps: adding N-Methyl-N'-nitro-Nnitrosoguanidine to a rhodobacter sphaeroides culture medium; and culturing the same at 15 Watts for 48 hours. The culture composition for promoting photosynthetic microorganism and animal cell growth includes a mutant strain of Rhodobacter sphaeroides, photosynthetic activating(or alternative) compound as an active ingredient. A manufacturing method of culture composition for promoting photosynthetic microorganism and animal cell growth comprises the following steps: a first step of manufacturing culture medium 1 by shake culturing the Rhodobacter sphaeroides at 30C for 48 hours; a second step of manufacturing culture medium 2 by mainly culturing the culture medium 1 at 30C for 48 hours; and a third step of manufacturing a culture composition containing Rhodobacter sphaeroides mutant strain, MBTLJ-8 by cultivating at 15 Watts for 48 hours after adding N-Methyl-N'-nitro-N-nitrosoguanidine to the culture medium 2.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Inoculate Rhodobacter sphaeroides into 5ml of sistrom's minimal medium and shake culture at 30C and 140rpm for 24 hours; (BB) Add 1ml of the culture medium into 100ml of the sistrom's minimal medium and main culture at 30C and 150rpm for 24 hours under the dark state; (CC) Add 1ml of the culture medium into 100ml of the sistrom's minimal medium and main culture at 30C and 150rpm for 24 hours under the light state; (DD) Add 1ml of the culture medium into 100ml of the sistrom's minimal medium, main culture at 30C and 150rpm for 12 hours under the 15W light state and for 12 hours under the dark state; (EE) Arrange the cultured cells and medium in a tube, centrifuge at 4 C and 5000rpm for 30 minutes, and collect precipitated cells; (FF) Adjust volume by adding sterilized water into the cells which are collected to be as much as 1/20 of the culture medium and sonicate using an ultra sonicator under a refrigerating state for 30 minutes based on the condition of 20 seconds pulse and 10 seconds recess; (GG) Name a solution obtained by filtering supernatant, which is centrifuged at 4C and 13000rpm for 30 minutes, using 0.2um filter as PACs