A TOLEROGENIC DENDRITIC CELL PREPARED BY USING VIRUS FOR TREATING MYOCARDITIS AND A PREPARATION METHOD THEREOF

摘要

PURPOSE: it is a kind of to prepare immunological tolerance Dendritic Cells Dendritic Cells and myocarditis provides to obtain specific immunological tolerance, inhibit the inflammation of myocarditis, effectively treatment myocarditis. ;CONSTITUTION: a kind of the step of preparing dendritic cell vaccination resistance treatment myocarditis includes: the heart in immature dendritic cell culture medium containing TNF-alpha and albumen by Coxsackie virus infection mouse. The protein extract, according to by homogeneous rat heart Coxsackie virus infection and centrifugation. The immature dendritic cell is prepared in dendritic cell precursor culture medium containing GM-CSF (the stimulation granular leukocyte macrophage colony factor) and IL-4. There is the immunological tolerance Dendritic Cells CD86 and CD40 of higher expression quantity to compare immature cell. ;The 2013 of copyright KIPO submissions