METHOD FOR SCREENING MATERIALS TO PREVENT OR TREAT OSTEOPOROSIS USING THE LEPTIN SECRETION CAPACITY OF MESENCHYMAL STEM CELLS

摘要

PURPOSE: A method for screening materials to prevent or treat osteoporosis is provided to select candidate materials to prevent or treat osteoporosis within a week.;CONSTITUTION: A method for screening materials to prevent or treat osteoporosis includes the following steps of: treating mesenchymal stem cells with a testing material, and culturing the treated material within a week; measuring the amount of leptin in cell the cultivated liquid by enzyme-linked anti body attaching reaction; and comparing the testing material with a control group, and selecting a candidate material which increases the amount of leptin. The testing material is selected from a group including synthetic compounds, microorganism cultured liquids or extracts, synthetic peptide, hexane, protein, antibody, aptamer, and natural extracts.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Control group; (BB) Dexamethasone 100nM; (CC,FF) IL-11 50 nanomole; (DD,GG) IL-17 50 nanomole; (EE) Adipogenesis control group; (HH) Mesenchyma stem cell; (II) Adipogenesis mesenchyma stem cell