TREATMENT OF AN IN VITRO CULTURED CELL LINE WITH AN ANTISENSE DNA OLIGOMER OF hsa-miR-106b miRNA AND A METHOD FOR CHANGING A SIGNAL TRANSDUCTION SYSTEM

摘要

PURPOSE: A treatment of an in vitro cultured cell line with an antisense DNA oligomer of hsa-miR-106b miRNA and a method for changing a signal transduction system are provided to show the same effect as endorphin by adding an antagomer or miRNA which has the same sequence as the antagomer using hsa-miR-106b miRNA.;CONSTITUTION: A method for promoting the expression of hsa-miR-106b target proteins comprises the step of treating an in vitro cultured cell line with an antisense DNA oligomer of hsa-miR-106b. The proteins are eukaryotic translation initiation factor 5A2, zinc finger/NFX1-type containing 1, FYVE and coiled-coil domain containing 1, polycystic kidney disease 2 (autosomal dominant), programmed cell death 1 ligand 2, cytochrome b reductase 1, pleckstrin homology domain containing- family A (phosphoinositide binding specific) member 3, myelin transcription factor 1-like, N-acyl phosphatidylethanolamine phospholipase D, and ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function). The expression level of the proteins is reduced by allowing hsa-miR-106b miRNA to target mRNA of a specific molecule.;COPYRIGHT KIPO 2013