PURPOSE: A kit for diagnosing HBe antigen-negative chronic hepatitis type B and liver diseases is provided to easily detect HBV-specific mutated HBV S antigen gene or protein. CONSTITUTION: A kit for detecting HBe antigen-negative chronic hepatitis type B contains a primer of sequence numbers 1-4. The kit contains: a DNA probe having base sequences of sequence numbers 11-18, which specifically binds to a gene encoding 100th amino acid of L110I S antigen protein; a linker which contains oligo(dT)15, -(CH2)6-, and amine group in order and is connected to thymine region at 5' terminal of the DNA probe; and a solid substrate having aldehyde which is attached on the surface, in which an amine group of an electric linker is connected to the aldehyde group by Schiff's base reaction.
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