METHOD OF DETECTING NON-HANWOO AND HANWOO USING DENATURING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY(DHPLC)

摘要

PURPOSE: A method for detecting mixture ratio of Hanwoo(Korean beef) and other beefs is provided to save time and cost for analysis and to determine quantitative ratio of the mixture. CONSTITUTION: A method for determine Hanwoo and other beefs comprises: a step of amplifying a mutation part of melanocortin 1 receptor(MC1R) gene of beef using a primer pair by PCR; a step of treating restriction enzyme; and a step of performing DHPLC(denaturing high performance liquid chromatography) of the treated gene. A forward primer contains partial or entire base sequence of sequence number 3. A reverse primer contains partial or entire base sequence of sequence number 4. The restriction enzyme is MspA1I or Msp1.