Mass spectrometry-based multiplex cytochrome P450 enzyme assay in biological samples

摘要

The invention of various kinds of activity of cytochrome P450 enzymes involved in the metabolism of steroid hormones in biological samples hydroxide using a mass analyzer at the same time the invention relates to analytical methods that can be measured and compared. The present invention can be measured at the same time the activity changes in the different types of cytochrome P450 enzymes in the metabolism of steroid hormones and related drugs according to a biological sample such as urine from a single pre-treatment and analysis as by indicating in a concentration ratio between its metabolites there. Therefore, the drug efficacy and metabolic mechanisms via the cytochrome P450 enzyme activity changes in specific drugs can be evaluated collectively, genome-based drug cytochrome P450 enzyme analysis and cross-validation (cross-validation) is also utilized as a possible analytical skills can. In addition, the scale of these information can be applied widely in the study of diseases related to the activity of cytochrome P450 enzymes as well as the development of new drugs and drug metabolism.