首页> 外国专利> Probes for identifying insecticide resistance mutations in the whitefly Bemisia tabaci, DNA chip, detection kit, and detection method using the same

Probes for identifying insecticide resistance mutations in the whitefly Bemisia tabaci, DNA chip, detection kit, and detection method using the same

机译:用于鉴定烟粉虱烟粉虱中抗药性突变的探针,DNA芯片,检测试剂盒和使用其的检测方法

摘要

The present invention is a gene chip, test kits, and Cigarettes oligonucleotide short for F392W mutation detection in Ace1 genes associated with M918V, L925I and T929V mutation detection and tolerance of organophosphate pesticides in vgsc gene using a nucleotide probe associated with Pierre droid resistance The present invention relates to a method for testing Louis insecticide resistance. The present invention is useful for detecting the resistance allele frequency in collected tobacco flour, and the specificity and sensitivity are almost 100% related to the determination of the insecticide resistance of tobacco flour, resulting in the determination of pesticide tolerance of tobacco flour. In this regard, it is possible to provide a method for discriminating tobacco powder pesticide tolerance mutations that is accurate and rapid and satisfies both specificity and sensitivity.
机译:本发明是与 vgsc中的M918V,L925I和T929V突变检测和有机磷酸酯农药耐受性相关的 Ace1 基因中的F392W突变检测的基因芯片,测试试剂盒和香烟寡核苷酸。使用与皮埃尔机器人抗性相关的核苷酸探针的基因技术领域本发明涉及测试路易杀虫剂抗性的方法。本发明可用于检测收集的烟草粉中的抗性等位基因频率,其特异性和灵敏度几乎与确定烟草粉的杀虫剂抗性有关,从而确定了烟草粉的杀虫剂耐受性。在这方面,有可能提供一种鉴别烟草粉农药耐受性突变的方法,该方法准确,快速并且满足特异性和敏感性。

著录项

  • 公开/公告号KR101267290B1

    专利类型

  • 公开/公告日2013-05-28

    原文格式PDF

  • 申请/专利权人 (주)지노첵;

    申请/专利号KR20100067473

  • 发明设计人 황승용;정인혁;정진욱;

    申请日2010-07-13

  • 分类号C12N15/11;C12Q1/68;

  • 国家 KR

  • 入库时间 2022-08-21 16:25:09

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