Detection method for metallothionein using quartz crystal microbalance immunosensor

摘要

The present invention comprises the steps of: (S1) a competitive reaction between the metallothionine immobilized on the electrode surface of the quartz crystal microbalance (QCM) chip and the sample against the primary antibody against metallothionine; (S2) reacting the secondary antibody tagged with HRP to the primary antibody bound to the immobilized metallothionine; (S3) adding a reaction buffer solution to the HRP-tagged secondary antibody bound to the primary antibody to obtain a frequency (F ₁ ) at a steady state; (S4) reacting the DAB / H ₂ O ₂ substrate solution with the HRP tagged secondary antibody bound to the primary antibody to obtain a frequency (F ₂ ) at steady state; And (S5) measuring a frequency change (ΔF = F ₁ -F ₂ ) from the frequency (F ₁ ) obtained in the step (S3) and the frequency (F ₂ ) obtained in the step (S4). The present invention relates to a method for detecting metallothionine, and according to the present invention, metallothionine contained in a sample can be detected with high sensitivity up to a concentration of 0.1 ng / ml.