A method for quantifying routine TLC

摘要

FIELD: chemistry.;SUBSTANCE: method for quantitative determination of rutin involves dissolving the analysed preparation sample while heating in ethanol, followed by chromatography using Sorbfil silica gel plates, eluent - ethyl acetate-glacial acetic acid-water (7.5:1.5:1.5); developer - 5% alcohol solution of NaOH; after developing the chromatographic zones, the plates are scanned by an EPSON PERFECTION 2480 PHOTO flatbed scanner and the obtained images are processed using Sorbfil Videodensitometer software, and content of rutin in the analysed sample (c, %) is calculated using the formulae: where S is the surface area of the chromatographic zone on the chromatogram, calculated using the Sorbfil Videodensitometre software.;EFFECT: rapidness, affordability, sufficient sensitivity, selectivity.;4 dwg