METHOD FOR PRODUCING ERYTHROCYTE antibody OVISNOGO DIAGNOSTICUM for the reaction of indirect hemagglutination (IHA) to indicate the OVISNOGO antigen in biomaterials

摘要

FIELD: biotechnologies.;SUBSTANCE: invention proposes a method for obtaining erythrocytic antibody diagnosticum for indirect hemagglutination reaction (IHAR) to indicate ovis antibody in biomaterial. The method is implemented by preparation of stabilised erythrocytes with further sensibilisation by positive ovis serum. In order to manufacture erythrocytic antibody diagnosticum, positive ovis serum for sensibilisation of stabilised erythrocytes is diluted in the ratio of 1:200-1:400, inactivated at 60°C during 30 minutes, and formalinised erythrocytes are sensibilised in the following ratio: one volume of 10% suspension of erythrocytes, one volume of positive ovis serum in the working dilution of (1:200-1:400), which is inactivated at 60°C during 30 minutes. Then, one volume of fresh water solution (0.1-0.11%) of water soluble alizarine blue indicator is added. Mixture is shaken and exposed during 60 minutes at 45°C, thus being stirred from time to time.;EFFECT: method allows obtaining erythrocytic ovis antibody diagnosticum having high activity and specificity.;1 tbl