Set of synthetic oligonucleotides for the detection of DNA of periodontal microorganisms TREPONEMA DENTICOLA by polymerase chain reaction

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to field of biochemistry, in particular to set of synthetic oligonucleotides for identification of DNA of parodontopathogenic microorganism Treponema denticola by method of polymerase chain reaction. Claimed set includes specific to fragment of gene licCA of microorganism Treponema denticola primers 5'-TAG CCG GAA AAA CGA AGG AGT G-3' and 5'-CCC TGC TTG TTT GCA AAC ATA G-3', as well as probe (BHQ1)-5'-AAC CCA GCC G(FdT)T TCG TCC TCC GAC-3'-P, where BHQ1 stands for attached to 5'-tail nucleotide blank fluorescence damper, FdT - fluorescent dye FAM, attached to T nucleotide.;EFFECT: claimed invention represents an effective marker for identification of microorganism Treponema denticola presence in biological material.