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METHOD FOR PROTECTION OF YEAST SACCHAROMYCES CEREVISIAE stressors DIFFERENT NATURE

机译:天然糖酵母酵母应激源的保护方法

摘要

FIELD: biotechnologies.;SUBSTANCE: method to protect yeast Saccharomyces cerevisiae against oxidising stress as a result of exposure to hydrogen peroxide includes growing of yeast culture under standard conditions till the end of the logarithmic or the start of the stationary phase of growth, incubation with a protective agent, exposure to hydrogen peroxide with subsequent determination of the number of survived cells. The protective agent is represented by soy inhibitors of proteases in concentration of 0.1-2.0 g/l. The time of incubation with the protective agent makes 5-6 hr, concentration of hydrogen peroxide is 700-750 mM. The invention provides for high extent of yeast cells production with no depressant action.;EFFECT: share of survived yeast cells during method realisation increases in comparison with the reference one 1,2-5,4 times.;7 ex
机译:领域:生物技术。;物质:保护酵母酿酒酵母免于因暴露于过氧化氢而产生的氧化应激的方法,包括在标准条件下生长酵母培养物,直到对数结束或生长的稳定期开始,然后与保护剂,将其暴露于过氧化氢中,然后确定存活细胞的数量。该保护剂以浓度为0.1-2.0g / l的蛋白酶的大豆抑制剂为代表。与保护剂一起孵育的时间为5-6小时,过氧化氢的浓度为700-750 mM。本发明提供了高水平的酵母细胞产生而没有抑制作用。效果:与参考文献相比,方法实现过程中存活的酵母细胞的份额增加了1,2-5,4倍。7 ex

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