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METHOD OF PREPARATION OF IN VITRO microshoots aspen, ash and willow rooting IN CONDITIONS OF EX VITRO
METHOD OF PREPARATION OF IN VITRO microshoots aspen, ash and willow rooting IN CONDITIONS OF EX VITRO
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机译:体外条件下微枝白杨,灰烬和柳树生根的制备方法
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摘要
FIELD: agriculture.SUBSTANCE: invention relates to the field of plant biotechnology and forestry. The invention is a method of in vitro preparing microshoots of ash, aspen, willow for subsequent rooting under ex vitro conditions, comprising the transfer of plants after the step of replication on a nutrient medium WPM for elongation, with the addition of sucrose 30 g/l, agar-agar 9 g/l, inositol 100 mg/l, pyridoxine 0.1 mg/l, thiamine 0.1 mg/l and nicotinic acid 0.5 mg/l, where glutamine is added to the medium for elongation at a concentration of 0.5 or 1.0 mmol/l, while culturing the plants is carried out with increased illumination intensity - from 5 to 8 thousand lux.EFFECT: invention enables to obtain physiologically and morphologically aligned shoots of aspen, ash and willow, which provides significant increase in the efficiency of their rooting ex vitro.4 tbl
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