SET AND METHOD FOR ACCELERATED PLAGUE MICROBE IDENTIFICATION AND SIMULTANEOUS DIFFERENTIATION OF VIRULENT AND AVIRULENT STRAINS OF YPESTIS BY PLASMID PROFILING THEREOF

摘要

FIELD: medicine.;SUBSTANCE: set involves primers and fluorescent-marked probes for 3a, cafl, pla, irp2, hmsH and IcrV gene sites within two different PCR mixtures. The set is used for the purpose of accelerated plague microbe identification and simultaneous differentiation of virulent and avirulent strains of Y.pestis by plasmid profiling. A method with the use of the set provides conducting multiplex PCR in two test tubes and test recording by probe mark signals.;EFFECT: invention enables higher diagnostic effectiveness of the strains.;2 cl, 8 dwg, 10 tbl, 1 ex