BACTERIOLOGICAL DIAGNOSTIC TECHNIQUE FOR Helicobacter pylori IN GASTRIC MUCOSA OF PATIENTS SUFFERING GASTRODUODENAL PATHOLOGY

摘要

FIELD: medicine.;SUBSTANCE: bacteriological diagnostic technique for Helicobacter pylori in gastric mucosa is implemented as follows. The examined material (biopsy material of gastric mucosa) is inoculated on Petri dishes containing standard medium of low sea water and agar. If small colourless colonies (of the diameter of 0.5-1.0 mm) with a smooth surface appeared to grow on the nutrient medium after 72-96 hours of incubation at temperature 36.0±1.0°C and high CO₂; the colonies are microscopically examined. If the smear occurred to contain thin Gram-variable rods or coccal forms, the grown colonies are examined for catalase formation, oxidase formation, urease formation, for an ability to degrade sodium hippurate, the absence of x factor (hemin consumption) and acid resistance (the absence of growth in thioglycollate medium at pH-3.5).;EFFECT: use of the invention reducing the length of examination and provides higher diagnostic reliability for Helicobacter pylori infection.;3 ex, 1 tbl