DIFFERENTIAL DIAGNOSTIC TECHNIQUE FOR LEIOMYOSARCOMA UTERI AND PROLIFERATING (CELL) LEIOMYOMATA

摘要

FIELD: medicine.SUBSTANCE: what is presented is a differential diagnosis technique for leiomyosarcomas uteri and proliferating (cell) leiomyomatas involving the analysis of molecular genetic variations of microsatellite DNA samples of tumour tissue. The genetic variations of the D17S1161, D10S1213, D10S1146, D10S218, D10S24, D9S942, D9S251 and D3S1295 microsatellites are to be found in the sample of tumour and surrounding normal tissue or peripheral venous blood by polymerase chain reaction (PCR) using specific primers. If observing some variations represented by heterozygosis loss and/or microsatellite instability in at least one of these microsatellites in the sample of tumour tissue, the malignancy is diagnosed.EFFECT: effective technique for differential diagnosis between leiomyosarcomas uteri and proliferating leiomyomatas by the presence of heterozygosis and microsatellite instability in the mentioned microsatellites in the sample of tumour tissue.2 dwg, 4 ex