METHOD OF ASSESSING EFFICIENCY OF IMMUNOMODULATOR IMUNOFAN APPLICATION IN EXPERIMENTAL CHOLERA

摘要

FIELD: medicine.;SUBSTANCE: invention relates to experimental medicine, in particular to study of immunomodulator efficiency for prevention and treatment of cholera. Before infection rabbits weighing 1.5-2.0 kg are given 10 injections of imunofan in dose 0.2 mcg, diluted in 0.3 ml of novocaine, every second day. After that animals are kept without food for 24 hours. After that, infection is performed by introduction of virulent strain of cholera vibrio into isolated loop of small intestine. Loops of small intestine are taken out, ligatures are applied, equal 12 cm long parts of intestine are tied up with 4-5 cm intervals between them under ether anesthesia. 1 ml of 0.9% sodium chloride solution is introduced into one ligated loop for control, with 10⁹ cells of 24-hour culture of Vibrio cholerae 5879 being introduced into another experimental loop. After that, abdominal wall is sutured and 18 hours later animals are killed for the further study and estimation of efficiency of imunofan application. Estimation is carried out by presence in experimental tied up loops of small intestine of enteropathogenic effect, whose expression is estimated in crosses, and presence of cholerogenic effect.The latter is calculated by formula: K=V_liq/L_loop, K is coefficient of loop stretching, V_liq is liquid volume, L_loop is loop length. If enteropathogenic effect is absent in experimental loops and K1.0, application of imunofan is considered to be efficient.;EFFECT: method ensures objective assessment of imunofan efficiency in case of cholera.;2 ex, 2 tbl