METHOD FOR FECES PREPARATION FOR DNA RECOVERY FOR PCR DIAGNOSING OF OPISTHORCHIASIS

摘要

FIELD: medicine.;SUBSTANCE: feces preparation for the DNA recovery is carried out by feces dissolution, homogenisation, filtration, fractionation, centrifugation and removal of a fecal tube. The feces are taken in an amount of 1000 mg, and a solvent is 0.1-1% acetic acid in an amount of 7 ml. The fractionation procedure is carried out using ethyl acetate in an amount of 2 ml, and the centrifuging is carried out at 1500 rpm for 1 minute.;EFFECT: invention improves the probability of PCT detection of opisthorchiasis even in the low content of opisthorchid fluke eggs in a clinical sample of faeces.;1 tbl, 1 ex