A method for sorting of at least one particles with a microfluidic sorting device with optical tweezers

摘要

A method for sorting of at least one particles comprising the steps of:The transport of a to be sorted sample solution containing particles (110) in a laminar samples solution current;Determining a geometric position and an optical classification of the to be sorted particle (110), wherein a position of an outer region of the particle to be sorted is identified;sequential sorting of the particle classified by means of targeted movement of the particle by means of a deflectable highly focused laser beam, which three-dimensional optical gradient exerts forces, from the detected position into a predetermined target position in the laminar samples solution current,the laser beam by means of a light microscope objective and focused by means of a scanner arrangement includes (115) is deflected in such a way that the particles to be sorted (110) within the channel (108) in a direction (113) is moved transversely to the flow direction (112) and transversely with respect to the beam axis of the laser beam by the flow cell (104) runs,the laser beam onto the outer region of the particle (110) to be sorted is focused,wherein the step..